Citrobacter freundii Lab Report Example | Topics and Well Written Essays - 250 words

Citrobacter freundii - Lab Report Example The plates were placed in an incubator and grown while observing the morphology. A Gram stain was performed on the unknown alongside quality control bacteria. The gram reaction was observed and recorded and specific biochemical tests performed. Given the reaction gave out a gram negative rod, the first test performed was oxidase test. Other tests performed are listed in the table found in the results section. After all the differential tests were performed, the results showed that the identity of unknown microorganism was citrobacter freundii. The results were compared against the class chart showing the classifications of the microorganisms. The determination that the unknown organism was gram negative rod initiated use of TSA slant to inoculate the other tests, Oxidase test, BCP lactose, Indole test, Citrate test, Motility test (Fraser, 76). All the results were well worked out for the the tests except for the citrate test which gave a false negative results. The error was noted given that, for this test, the result was inconsistent with other tests. The test was repeated and was able to give a positive result. In conclusion, the unknown organism was found to be Citrobacter freundii, following a series of eliminations made from the tests. Citrobacter freundii is in the Enterobacteriaceae family and are gram-negative bacilli (Fraser p. 20). It is normally found in sewage and intestinal tract of animals and humans. It can cause disease to its