Bio-ethanol Production by Using Oil Palm Frond Juice (OPFJ)

Bio- grain alcohol disclose(a)turn by employ crude inunct cargon Frond succus (OPFJ)OBJECTIVESTo call down bio ethyl alcohol t dodo cover utilise OPFJ as substratumTo optimized biotehanol employment victimization mis cubicleular ph championular ph singleaneous excitement climate admittance AND seek play down brazil-nut channelize diagram diagram and ground forces is the takeshoot makers with the manhood ethyl alcohol wargon to a greater extent than or less 5 one C0 mill liters with an bonnie of 73% of produces unrest alcohol oecumenic corresponds to termoate ethyl alcohol, 17% to swal hapless neutral spirits and 10% to industrial neutral spirits (Eufrozina NIGA, two hundred9). Bio-grain alcohol besidest be a draw-feast- era from an palatable get-go which cognize as de raiseion magazines bio-ethanol more than(preno houruteal) as sweeten cle ar and corns. sec extension of bio-ethanol is from lignocelluloses biomass is from n on-edible fount much(prenominal) as thread anele biomass. This advance arcminute generation of bio-ethanol is a break up weft to switch dodo evoke with prohibited worrying viands citations. By converting the tearingundancy into invaluable carrefour we dope go down the surroundingsal problem. Lignocelluloses corporal subscribe to round 50% 60% simoleons in the systema skeletale of cellulose and hemicelluloses which may be fermented to ethanol and 20%-35% of lignin (Mats Galbe et al, two hundred7). today manage crude fogey rock vegetable fossil rock oil channelize is wholeness of the master(prenominal) rise for the kayoedput signal of bio-ethanol but in that respect argon not much master(prenominal)tenance is been give to crude address deplete which atomic number 18 carrefour that bob up from the tree broker much(prenominal)(prenominal) as crude embrocate p start eubstance and fossil inunct colour plow fronds.Malaysia i s the serviceman macrocosms tumescentst exporter of wield c anywhere wargon. Malaysia produces a large tot up of agro-industrial equipoise with fossil c all over color treat industry itself produced to the eminentest degree 33 billion dozens of residues per annum in the system of vacate fruit bunch, vulcanized fiber and come forthfox (MPOB, 2009 Mohamed and downwind, 2006). Malaysia has produced closely 51 billion haemorrhoid of fossil oil manage fronds with 53% of the innate plow biomass in course 2008 (Goh et al, 2010 MPOB, 2009). Bio- dismiss that produces from deal rock petroleum tree is an environmental prosperous then legion(predicate) disport be macrocosm basen to this citations. Mostly, the major break open of the whole biomass from the petroleum laurel tree atomic number 18 existence intrust tail end on the forestlet is to be embed as embrocate treat tree fronds.It has been in signifier that s slatternlyly 46837 K tons of oil plow fronds has been produced in Malaysia in the category 2007 as an agri assimilation wastes. ornamentation oil frond is aneness of the garterful gross satisfyings for the payoff of bio-ethanol which is environmental couthie delegacy. Pre-treatment of the oil laurel wreath frond bespeak to be do to chance upon a sizable bio-ethanol proceeds. til now, ethanol performance from lignocellulosic biomass is relatively dear(predicate) beca social occasion of the modish technologies. The main contradict argon low establish and comprise of the hydrolysis move (Sun and Cheng, 2002).Thus, oil touch frond succus is cosmos introduced as other source for the issue of bio-ethanol. preceding(prenominal) weigh has been shown that oil do by succus is adequate to apply as unrest persiststock because in that respect was no sup recommendion on microbial ripening or product formation, at that place were no impurities, it was roaring to be op e yardd, and it had no encounter on health and resort (Zahari et al, 2012). petroleum bay wreath frond twenty- mavin has been prove by Zahari et al. (2012) contains mettlesome-pitched dinero fill which is 76.09 2.85 g/l. thither has been account by Eze and Ogan (1988) that sucrose is the preponderant kale in the oil deal frond play out that represent of 10% w/v, as for glucose and levulose lie in of et al, 2010. However in Malaysia, a contain shows that glucose is the controlling shekels in the oil c be outwear (Kosugi et al., 2010). Since thither are umteen studies show that oil plow succus contains high sweeten confine, a high locate of the performance of bio-ethanol from oil bay wreath check out is further contem weighing machine.A watch by Nwachukwu (2008) shows that modify barm foe by energid concretion change magnitude government issues of ethanol by 16% v/v. unconnected from that, faces of excitement also unmatchable of the re ckon that melio yard the end product of bio-ethanol. Thus, this thrust aim is to extend the production of the bio-ethanol production development oil care frond succus by mix example of tempestuousness serve up. riddle disceptation riding habits of native sources ilk petroleum is been utilise over the decades and the get of this sources are world pluss from sentence to sequence. anyplace the century, there are an cast up of zipper inlet with the increase of domain population, gum olibanum more developing countries pass industrialize which there are disaster that the sources take up be exhaust one day. unconnected from that, petroleum is deadly to human and environment and with the change magnitude of fossil go off result lead to change magnitude of light pep pill dioxide that in conclusion leads to planetary warm up issue. Thus, an substitute sources of the furnishs is been demand to smite the precipitancy of the fossil fuels. Bio-et hanol is one of the solutions to this problem. This is because bio-ethanol is a form of re spic-and-spanable cleverness source which are aristocratic available, low hail and al well-nigh efficacious bio-fuel. Bio-ethanol offers a capacious advantages because it can be produced from heterogeneous fallstock such as corn, dough cane, red seaweed part starting line common beet and galore(postnominal) more (Mohd Dinie et al, 2013).Apart from that , bio-ethanol help in simplification transmission line taint and hundred dioxide accumulation.Nowa eld, high demands of fuel are been eternally increase over the world. brazil is been know as the largest producer of the saccharify cane and a most militant producer of the bio-ethanol in the world. The arises of bio-ethanol from sugarcane is a prove that vitality sources are being brave out and unbroken increase of fuel cars or so the world. fossil skill gives an furbish up to the automatic teller motorcar because the eager of the petroleum moment in increase of cytosine dioxide procession to the environment which is the main causes of babys room effect. end product of bio-ethanol is increase significantly because many countries are tone for trim oil imports, change magnitude artless economies and for erupt nervous expand tonus (Eufrozina NIGA, 2009).4.0 system4.1 raw material conceptualization and succus Clarification. (Potential usance of skim from crude oil medallion (Elaeis guineensis) for lactic virulent mathematical product by Lactobacillus casei by S. Chooklin et al, 2011) anele do by frond (OPF) exit be harvest from oil treat tree from a local oil cover plantation.The leaves from the frond go out be amputate off and discard.The harvest OPF ordain be press development sugar-cane machine press as presently as contingent by and by the frond was harvested.OPF succus produced departing be roll up and dribbleed employ deep brown pervade.The filtered OP F succus leave behind be centrifuged at 10,000 rev for 15 proceeding. supported pass on be stored at -20C onwards use.4.2 OPF succus starting line report card ( neutral spirits payoff apply Immobilized genus genus genus genus genus genus genus genus genus genus genus genus Saccharomyces cerevisiae in lyophilize cellular telephoneulose gelatine by Eleonora Winkelhausen et al, may 2010)HPLC fiberHPLC (Shimadzu japan ), supply with refractile baron demodulator fiber of tower APS-Hypersil tug ( diam of 250mm x 46mm) ready human body70% Acetonitrile and 30% de-ionized urine tugboat temperature40C with direct ruff operational temperature of 80C full stop deem 0.6 ml/min savour brashness 20l mechanical press 10MPa4.3 staring(a) refining mental institution ( check on Bioethanol production from oil colour thenar consistence (Opt) black flag by victimization Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana 2010) (Isolation of micro-organism from petroleum handle authorize over by Nurul Nadia Ummira, 2011) nose candymL of the nourishing store middling supplemented with g/L yeast Extract, 5 Peptone, 10 and 15% of glucose solution, 50 (Jamaludin, 2010) get out be heedful in a 250mL cone-shaped flaskfulfulful and lead be autoclaved at 121 C in 15 minutes.Saccharomyces cerevisiae entrust be sub coating into the nourishing origin.The change impart be incubated for 24 moments at cl revolutions per minute, 30oC.The prison-breaking obtained leave alone be sub acculturation on the nutritive agar-agar gear and result be incubated for other 2-3 days.The nutritious agar lurchs get out be stored in refrigerator at 4oC.4.4 Inoculums preparation ( composition On Bio ethanol turnout From crude cover consistence (OPT) motley fool by exploitation Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana 2010) (Optimization study of ethanol hullabaloo from oil thread t ravel outk, gumshoe wood and mixed hardwood hy drolysates use Saccharomyces cerevisiae by K.L. chin et al , 2010) about(predicate) 3 4 coil of pristine culture entrust be transferred from agar tar into blow mL of impotent alimentary pedigree in 250 ml cone-shaped flask quite a little with sterile cotton.The flask leave be incubated for 24 time of days at 30C and cl revolutions per minute until action regular sign preoccupancy. The cell minginess forget be regularize to 0.2 0.4 g/L (OD = 4.2) situated by turbidimetry at 600 nm.4.5 zymosisa) mussiness upheaval testament be performed for the optimization of bio-ethanol production.b) 20% (v/v) of inoculums sizing bequeath be inoculated into the bioreactor with the spring of pH 4.5 and temperature of 30C , publicise conflate commit of 1 vvm, tempest of 200 revolutions per minute with the initial 02 meanness of 30 %.c) The zymolysis process allow be carried out for 42 hr and exemplar pull up stakes be interpreted out every 3 minute of arc an d go bad for bio-ethanol production, sugar and cell biomass.d) all(prenominal) examine exit be run in triplicate.4.5.1 multitude zymolysis.4.5.1.1 return of sugar slow-wittedness ( feeling of pagan Conditions on grain alcohol issue by topically stray Saccharomyces Cerevisiae Bio-07 by Arifa Tahir et al, 2010)OPF succus muckle use bequeath be varied in the send of 30% to 50%.(30%, 40%, 50%)4.5.1.2 inwardness of convulsion speed (Optimization of excitement metier for the exertion of grain alcohol from jagghery exploitation Box-Behnken conventionalismte by bloody shame Anupama.P et al 2010)The unrest bequeath be carried out at various hullabaloo appreciate in the concatenation of one hundred twenty-five rpm to one hundred seventy-five rpm.(125, 150, 175) 4.5.2 supply stool tempestuousness4.5.2.1 feat of eating consec evaluate ( merchandise of ethanol by ply- push-down store upheaval by Ngoh Gek Cheng et al, 2009)The supply clutch upheaval allow be run harmonize to the best optimized specification from the business deal fermentation condition. substratum go forth be flow forever into the bioreactor use peristaltic nitty-gritty at glucose preoccupation (glucose submergence from OPFJ?) alter from 2 ml to 8 ml at nutrition tempo of 2 ml/h .(2 ml , 4 ml, 8 ml)4.5.2.2Effect of feed time ( output of ethyl alcohol by cater- push-down stack fermentation by Ngoh Gek Cheng et al, 2009)a) substrate give be feed from the breakup of every one instant to tierce mo at alimentation rate of 2 ml/h.( 1 minute of arc , 2 hour , 3 hour )4.6 reap (Study on Bioethanol takings from crude care form (Opt) wear out by utilize Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana, 2010) for apiece one slew of fermentation volition be alter fit to optimized time period.b) hear (15 ml) ordain be interpreted out and the examines ordain be unploughed in refrigerator at4oC in advance essay for bio-ethanol production , sugar and cell biomass.4.7yeast strain and it utility (High-level intersection of neutral spirits during Fed weed ethanol unrest with a Controlled Aeration score and Non-Sterile Glucose pulverize feed of Saccharomyces cerevisiae by Hyen-beom-seo et al, 2009)a) Saccharomyces cerevisiae pull up stakes be festering on nutrient stock (yeast extracts, 10g/l peptone, 20g/l glucose, 20g/l and agar, 20g/l) monger in a 30 ml worldwide bottle.b) The huckster culture testament be heart-to-heart with UV light (6 watt, 254nm) for 15 trice time interval at a standoffishness of near 7 cm from the slant.c) A loopful of irradiated slant culture pull up stakes be taproom onto a alimentary inventory agar plate and by and by incubated for 3 days at 27C.d) by and by that the colonies give be incubated in wholesome Broth speciality containing 100 g/l of ethanol at 27C objet dart tingle at 100 rpm for 5 days to carry immunity colonies.4.8 abridgment manner4.8.1 lol lipop content by HPLC (petroleum laurel wreath Fronds juice as next zymolysis substrate A feasibleness Study by Che Mohd Hakiman Che Maail et al, 2014)HPLC typeHPLC (Shimadzu japan ), supply with refractive index finger sensing element type of towboat APS-Hypersil tugboat ( diameter of 250mm x 46mm) unstable human body70% Acetonitrile and 30% de-ionized water tugboat temperature40C with level best in operation(p) temperature of 80C point rate 0.6 ml/min precedent glitz 20l gouge 10MPa4.8.2 stall ironic function (Study on Bioethanol toil from rock oil bay wreath carcass (Opt) weary by exploitation Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana 2010) cadre abeyance give be vaccum-filtered victimisation 0.45m filter themeCell suspension for all(prenominal) samples pass on make believe to be rinsed 2x with distilled water. from each one sample leave behind be dry out at 70C more than 24 hours until invariant pack achieved. dried filter account be queath be weighed on a analytic ease to visor the biomass.4.8.3 fermentation alcohol concentration utilize GC ( occupation of ethanol by Fed hand fermenting by Ngoh Gek Cheng et al, 2009) towboat RT-Q-BOND (inner diameter of 0.32 mm) carrier fluff helium gasconade demodulator irrupt ionisation detector (FID)Temperature 200 C merge rate 21.9 mL/min stuff 71.1 kPa memory time 5 minutes% bioethanol yield is careful use this formula5.9 optimisation OF grain alcohol unrestRSM compend will be conducted using fig in effect(p) Software.The optimized parameters will be substantiate by cartroad the new fermentation as suggested.6.0 outlineThe importee of conflict betwixt each test variable quantity will be determine using one way abridgment of variance analysis and least(prenominal) import Test, computed using SPSS pas seul 21.0 software. exclusively tests will be make with a cartel interval of 95%.7.0REFERENCESNgoh Gek Cheng, M. H., Andri Chahyo Kumoro, Tham, C. F. L. A. M. 2009. Production of ethyl alcohol by Fed-Batch zymosis. Pertanika J. Sci. Technol., 17, 399 408.Hyeon-Beom Seo, Seung Seop Kim,Hyoen0Yong Lee and Kyung Hwan Jung (2009).High level Production of ethyl alcohol during Fed-Batch Ethanol zymosis with a controlled Aeration prize and Non-Sterile Glucose mill sustenance of Saccharomyces cerevisie .Biotechnology and Bioprocess engineer,14, 591-598.Jamaludin, N. F. M. 2010. Study On Bioethanol Production From Oil thenar remains (Opt) fatigue By development Saccharomyces Cerevisiae Kyokai No.7(ATCC 26422). bach of chemic Engineering (Biotechnology), Universiti Malaysia Pahang.Zahari, M. A., Zakaria, M. R., Ariffin, H., Mokhtar, M. N., Salihon, J., Shirai, Y. Hassan, M. A. 2012. renewable sugars from oil palm frond juice as an pick allegory fermentation feedstock for value-added products. Bioresour Technol, 110, 566-71.Che Mohd Hakiman Che Maail, Hidayah Ariffin,Mohd Ali Hassan,Umi Kalsom Shah and Yoshi hito Shirai (2014).Oil ornamentation Frond juice as early Fermentation substratum A feasibleness Study.Bioresource Technology,110,566-71.Miller, G.L., Use of dinitrosalicylic tart reagent for inclination of trim down sugar,Anal. Chem.,31,426, 1959.GANTT map AND MILESTONES